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The Draft Genome And Transcriptome Of Cannabis Sativa

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작성자 Erlinda 작성일23-07-31 00:05 조회2회 댓글0건

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The final merged assembly contains 40,224 transcripts falling into 30,074 clusters of isoforms (Table 3). We selected the transcript with the most important open studying frame (ORF) because the representative for each cluster, resulting in a pruned assembly with an N50 of 1.91 kb. PK transcripts are partial or that genes are represented by multiple non-contiguous fragments. We famous a 3' end bias within the normalized cDNA library, presumably as a result of polyA priming step (knowledge not shown). Moreover, by combining near-an identical transcripts during meeting merging and isoform clustering, we doubtless collapsed transcripts of massive multi-copy gene families. Indeed, applying our isoform clustering algorithm to the Arabidopsis meeting reduces the full number of bases to 44 Mb, which is usually due to the loss of transposable factor genes. Overall, our assembled PK transcriptome is therefore very similar to the deeply characterized Arabidopsis transcriptome, each in measurement and composition. Transcript lessons in Cannabis sativa and Arabidopsis thaliana.

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